市场上大部分基于表型鉴定的细菌鉴定分析系统普遍需要做革兰氏染色等前处理，容易选错鉴定板而无法获 得准确结果。美国BIOLOG公司凭借其高通量表型芯片平台，优选出71种碳源和23种化学敏感物质，首次将革 兰氏阴性细菌和阳性细菌用同一块板进行鉴定，大大简化鉴定步骤，增加了数据库的扩展性，提高鉴定灵敏度和 准确性。

1.主要用途：用于各种与微生物相关的领域包括制药、食品、化妆品等常见的好氧细菌、厌氧菌、酵母和霉菌等微生物的鉴定。

2.鉴定原理：以碳源利用及化学物质敏感性检测为原理，用于鉴定的反应数量95种。

3.工作条件：

3.1电源要求: 220-240V AC（交流），50Hz

3.2环境温度：18℃—30℃

3.3相对湿度：20%—85%

4.仪器技术指标：

4.1系统数据库部分：（Biolog厂家会随时根据国际微生物命名变更而更新数据库）

4.1.1数据库容量大于2990种(taxa)，标配包含四大类数据库，其中好氧细菌数据库1570种（革兰氏阴性好氧菌800种，革兰氏阳性好氧菌770种），厌氧菌393种，酵母菌313种，丝状真菌715种。几乎涵盖了所有常见的人类、动物、植物病原菌以及食品、药品和环境微生物。

4.1.2 BIOLOG丝状真菌数据库（FF），可自动鉴定包括临床、工业、农业及环境中常见青霉（120种）、曲霉（105种）、刺盘孢霉（21种）、镰刀霉（68种）、木霉（45种）及其它种等715种（taxa）丝状真菌。为与传统的真菌形态学鉴定相结合，BIOLOG提供近1000多张精美的宏观和显微图片供用户参考比对，以获得更为精确的结果。

4.1.3 BIOLOG在食品、化妆品和制药工业领域提供大量的常见致病菌数据库，包括沙门氏菌（15种）、李斯特菌（8种）、大肠杆菌（7种，含阪崎肠杆菌和E.coli O157:H7）、葡萄球菌（43种好氧，2种厌氧）、弯曲菌4种、假单孢菌（130种）、弧菌（24种）、厌氧梭菌（46种）、志贺氏菌（4种）、耶尔森氏菌（14种）、链球菌（74种，含猪链球菌）、金黄杆菌属（32种）等，用于食品、药品及化妆品行业致病微生物鉴定、工业应用微生物研究、质量控制及产品研发等。

4.1.4 BIOLOG在植物病原菌鉴定方面，可鉴定常见的植物病原菌达到约258种，如假单孢菌 （130种），伯克霍尔德菌（13种）、黄单孢菌 属（81种）、果胶杆菌属（14种）、食酸菌属 （8种）、短小杆菌（9种）、根瘤菌属（4种）、欧文氏解淀粉菌等，数百种植物致病细菌及数百种的植物病原真菌，提及Biolog鉴定方法的国标GB/T 10项，海关行业标准SN/T 7项。

4.1.5 Biolog数据库包含常见的331种兽医微生物，例如14种巴氏杆菌、24种奈瑟氏菌、14种莫拉杆菌、64种诺卡氏菌、24种弧菌、16种放线杆菌、19种气单孢菌及其它各种常见兽医微生物。

4.1.6 可鉴定23种分枝杆菌(Mycobacterium)。

4.1.7 可鉴定放线菌属共19种，其中革兰氏阳性好氧放线菌7种，厌氧放线菌12种。

4.1.8 可鉴定芽孢杆菌89种（株）。

4.1.9 可鉴定51种乳酸杆菌（好氧34、厌氧17）、30种厌氧双歧杆菌。

4.2系统操作性能：

4.2.1好氧细菌使用Gen III鉴定板，无需进行革兰氏染色、氧化酶和三糖铁等预测试工作，直接将培养好的纯种配制菌悬液后定量接种至鉴定板即可。

4.2.2鉴定时间：细菌2－22小时，酵母24－72小时，霉菌3－7天。

4.2.3系统维护保养简单，维护成本低。

4.3系统软件功能：

4.3.1可生成用户自定义数据库。构建系统数据库之外新发现的菌株数据库，便于将来菌株的聚类分析和溯源分析。

4.3.2 用于鉴定的反应数量多达95种，鉴定结果特异性强、分离度大、扩充空间巨大。

4.3.3 独创的算法分析模型，用相似性（SIM）和距离 （DIS）等参数来判定鉴定结果，综合考虑了结果与标准数据库的匹配程度及距离大小，更加真实地反应了鉴定结果的好坏。

4.3.4 数据管理软件模块，用户可对数据文件进行备份、筛选、合并、浏览、生成聚类图、编辑及输入或输出。

4.3.5可选数据追溯模块，数据安全性符合美国FDA的21CFR PART11要求。

4.4系统硬件部分

4.4.1读数仪：美国原产光栅式微孔板读数仪，常用波长490nm,590nm和750nm。

4.4.2浊度计：OD值和透光率双刻度指针式显示，电池供电。

4.5 权威认可(共17个标准，其中推荐国标10个，推荐行标7个)

GB/T 36840-2018 玉米内州萎蔫病菌检疫鉴定方法

GB/T 28075-2011 萨氏假单胞杆菌菜豆生致病型检疫鉴定方法

GB/T 28096-2011木薯细菌性萎蔫病菌检疫鉴定方法

GB/T 28099-2011 水稻细菌性条斑病菌的检疫鉴定方法

GB/T 28106-2011 郁金香黄色疱斑病菌检疫鉴定方法

GB/T 29396-2012 水稻细菌性谷枯病菌检疫鉴定方法

GB/T 36844-2018十字花科细菌性黑斑病菌检疫鉴定方法

GB/T 28978 2012马铃薯环腐病菌检疫鉴定方法

GB/T 29578-2013 日蔗白色条纹病菌的检疫鉴定方法

GB/T 36847-2018兰花褐斑病菌检疫鉴定方法

SN/T 4730-2016 菊基腐病菌检疫鉴定方法

SN/T1135.9-2010 马铃薯青枯病菌检疫鉴定方法

SN/T 2601-2010 植物病原细菌常规检测规范

SN/T 2736-2010核果树溃疡病菌检疫鉴定方法

SN/T 1135.9-2010 马铃薯青枯病菌检疫鉴定方法

SN/T 1813-2006蝴蝶兰细菌性软腐病菌检疫鉴定方法

SN/T 2602-2010根癌土壤杆菌的检测鉴定方法

5.系统配置：

5.1整套主机包含微孔板读数仪1台、浊度计1台、鉴定基础软件1套、四个数据库1套。

5.2可选耗材：鉴定板（用于菌种的筛选），培养基，接种液，其它耗材等。

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16. Stenothermobacter spongiae gen. nov., sp. nov., a Novel Member of the Family Flavobacteriaceae Isolated from a Marine Sponge in the Bahamas, and Emended Description of Nonlabens tegetincola, S.C.K. Lau, M.M.Y. Tsoi, X. Li, I. Plakhotnikova, S. Dobretsov, M. Wu, P-K. Wong, J.R. Pawlik, and P-Y. Qian, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 181-185.

15. Shewanella halifaxensis sp. nov., a Novel Obligately Respiratory and Denitrifying Psychrophile, J-S. Zhao, D. Manno, C. Leggiadro, D. O’Neil, and J. Hawari, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 205-212.

14. Myceligenerans crystallogenes sp. nov., Isolated from Roman Catacombs, I. Groth, P. Schumann, B. Schutze, J.M. Gonzalez, L. Laiz, M-L. Suihko, and E. Stackebrandt, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 283-287.

13. Krokinobacter gen. nov., with Three Novel Species, in the Family Flavobacteriacea, S.T. Khan, Y. Nakagawa, and S. Harayama, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 323-328.

12. Methylobacterium adhaesivum sp. nov., a Methylotrophic Bacterium Isolated from Drinking Water, V. Gallego, M.T. Garcia, and A. Ventosa, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 339-342.

11. Pseudovibrio ascidiaceicola sp. nov., Isolated from Ascidians (Sea Squirts), Y. Fukunaga, M. Kurahashi, K. Tanaka, K. Yanagi, A. Yokota, and S. Harayama, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 343-347.

10. Bacillus macauensis sp. nov., a Long-Chain Bacterium Isolated from a Drinking Water Supply, T. Zhang, X. Fan, S. Hanada, Y. Kamagata, and H.P. Fang, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 349-353.

9. Thalassomonas loyana sp. nov., a Causative Agent of the White Plague-Like Disease of Corals on the Eilat Coral Reef, F.L. Thompson, Y. Barash, T. Sawabe, G. Sharon, J. Swings, and E. Rosenberg, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 365-368.

8. Patulibacter minatonensis gen. nov., sp. nov., a Novel Actinobacterium Isolated Using an Agar Medium Supplemented with Superoxide Dismutase, and Proposal of Patulibacteraceae fam. nov., Y. Takahashi, A. Matsumoto, K. Morisaki, and S. Omura, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 401-406.

7. Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., Isolated from Roots of Sand-Dune Plants, M.S. Park, S.R. Jung, K.H. Lee, M-S. Lee, J.O. Do, S.B. Kim, and K.S. Bae, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 433-438.

6. Acaricomes phytoseiuli gen. nov., sp. nov., Isolated from the Predatory Mite Phytoseiulus persimilis, R. Pukall, P. Schumann, C. Schütte, R. Gols, and M. Dicke, International Journal of Systematic and Evolutionary Microbiology, 2006, v.56, pp. 465-469.

5. Biolog: Modern Phenotypic Microbial Identification, B.R. Bochner, Encyclopedia of Rapid Microbiological Methods, 2006, v.2, Ch. 3, pp. 55-73.

4. Description of Xenorhabdus khoisanae sp. nov., the symbiont of the entomopathogenic nematode Steinernema khoisanae, Tiarin Ferreira, Carol A. van Reenen, Akihito Endo, Cathrin Spröer, Antoinette P. Malan and Leon M. T. Dicks, International Journal of Systematic and Evolutionary Microiology, March 2013

3. Efficacy of native antagonistic bacterial isolates in biological control of crown gall disease in Egypt, I.H. Tolba, M.A. Soliman, Annals of Agricultural Sciences, March 2013

2. Antimicrobial Resistance in Enterococcus spp. Isolated from Environmental Samples in an Area of Intensive Poultry Production, Vesna Furtula, Charlene R. Jackson, Erin Gwenn Farrell, John B. Barrett, Lari M. Hiott and Patricia A. Chambers, International Journal of Environmental Research Public Health, 2013

1. Phylogenetic signal in phenotypic traits related to carbon source assimilation and chemical sensitivity in Acinetobacter species, A. Assche, S. Álvarez-Pérez, A. Breij, J. Brabanter, K. Willems, L. Dijkshoorn, B. Lievens, Applied Microbiol Biotechnology, October 2016